Description
This assay employs a two-site sandwich ELISA to quantitate HSP-70 in samples. An antibody specific for HSP-70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSP-70 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSP-70 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP-70 bound in the initial step. The color development is stopped and the intensity of the color is measured